Cell Biolabss：氧自由基抗氧化能力（ORAC）活性檢測試劑盒

自由基與多種人類疾病的發生發展都有著密切的關系。在清除自由基的過程中，抗氧化劑發揮著重要的作用。因此，了解抗氧化劑的作用及能力就變得至關重要。

在評價抗氧化劑能力的諸多方法中，抗氧化能力ORAC（又稱，氧自由基吸收能力，Oxygen Radical Absorbance Capacity）分析法，被認定為檢測抗氧化劑抗氧化能力的標準方法，是目前普遍被接受的一個標準評價手段。由于該法具有接近機體生理條件、操作簡單、認可度和靈敏度高、準確性和重現性佳、高通量、不易受人為因素干擾等優點，被廣泛用于農業，食品，保健品，藥品及醫學領域。

那么如何分析樣本中氧自由基抗氧化能力/氧自由基吸收能力（ORAC）？作為專業的生命科學醫藥原料供應商，艾美捷科技為您推薦：眾多文章引用的，氧自由基抗氧化能力（ORAC）活性檢測試劑盒

* 本產品僅適用于科研用途.

氧自由基抗氧化能力（ORAC）活性檢測試劑盒，原理與標曲展示：

氧自由基抗氧化能力（ORAC）活性檢測試劑盒-原理示意圖

氧自由基抗氧化能力（ORAC）活性檢測試劑盒-標曲示意圖

FAQ：

1.是否可以測試冷凍保存的樣品？

RE：通常適用于在 -80℃ 下保存兩個月以內樣品，但我們建議盡可能測試新鮮樣品以獲得最佳結果

2.ORAC、HORAC和TAC檢測之間的區別，如何選擇？

RE：所有三種測定都測量抗氧化能力，但略有不同。ORAC檢測分析氧自由基的抗氧化能力；HORAC檢測分析羥基自由基的抗氧化能力；TAC基于單電子轉移(SET)機制和銅離子，檢測總體抗氧化能力?？傮w而言，研究人員更偏好選擇ORAC分析而不是HORAC，因為過氧自由基似乎比羥基自由基更相關。

3.更多常見問題，請點擊：cellbiolabs.com/faq/oxidative-stress-faq/orac-assay

近期發表文章（僅摘取部分展示）：

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Yuasa, M. et al. (2020). Antioxidant and taste properties of fresh onion (Allium cepa L.) leaves. J. Food Meas. Charact. doi: 10.1007/s11694-020-00704-w.

Wake, H. et al. (2020). Histidine-rich glycoprotein possesses anti-oxidant activity through self-oxidation and inhibition of hydroxyl radical production via chelating divalent metal ions in Fenton's reaction. Free Radic Res. doi: 10.1080/10715762.2020.1825703.

Suruga, K. et al. (2020). Soybean fermentation with basidiomycetes (medicinal mushroom mycelia). Chem. Biol. Technol. Agric. 7:23. doi: 10.1186/s40538-020-00189-1.

Cheng, H.S. et al. (2020). Pleiotrosepic ameliorative effects of ellagitannin geraniin against metabolic syndrome induced by high-fat diet in rats. Nutrition. doi: 10.1016/j.nut.2020.110973.

Jeon, S.Y. et al. (2020). Combined Extract of Vitis vinifera L. and Centella asiatica Synergistically Attenuates Oxidative Damage Induced by Hydrogen Peroxide in Human Umbilical Vein Endothelial Cells. Prev Nutr Food Sci. 25(2):173-183. doi: 10.3746/pnf.2020.25.2.173.

Xiao, L. et al. (2020). Enzyme-digested Colla Corii Asini (E’jiao) prevents hydrogen peroxide-induced cell death and accelerates amyloid beta clearance in neuronal-like PC12 cells. Neural Regen Res. 15:2270-2. doi: 10.4103/1673-5374.285000.

Yang, Y. et al. (2020). Chemical and cytological evaluation of honeybee pollen antioxidant ability. J Food Sci. doi: 10.1111/1750-3841.15047.

Hu, R. et al. (2020). Antioxidant Performances of Corn Gluten Meal and DDGS Protein Hydrolysates in Food, Pet Food, and Feed Systems. Journal of Agriculture and Food Research. doi: 10.1016/j.jafr.2020.100030.

Liu, Y.H. et al. (2020).  Acetylcholinesterase inhibitory activity and neuroprotection in vitro, molecular docking, and improved learning and memory functions of demethylcurcumin in scopolamine-induced amnesia ICR mice. Food Funct. doi: 10.1039/C9FO02339A.

Kim, D. et al. (2020). Pig Skin Gelatin Hydrolysates Attenuate Acetylcholine Esterase Activity and Scopolamine-induced Impairment of Memory and Learning Ability of Mice. Food Sci Anim Resour. doi: 10.5851/kosfa.2020.e3.

Mishima, E. et al. (2019). Drugs Repurposed as Antiferroptosis Agents Suppress Organ Damage, Including AKI, by Functioning as Lipid Peroxyl Radical Scavengers. J Am Soc Nephrol. pii: ASN.2019060570. doi: 10.1681/ASN.2019060570.

Riquelme, S. et al. (2019). Bench-scale extraction of stilbenoids and other phenolics from stored grape canes (Vitis Vinifera):  Optimization process, chemical characterization, and potential protection against oxidative damage.  Journal of the Chilean Chemical Society. 64(2).

Liu, Y.H. et al. (2019). Anti-glycation, anti-hemolysis, and ORAC activities of demethylcurcumin and tetrahydroxycurcumin in vitro and reductions of oxidative stress in D-galactose-induced BALB/c mice in vivo. Bot Stud. 60(1):9. doi: 10.1186/s40529-019-0258-x.

Engstr m- st, J. et al. (2019). Eco-physiological responses of copepods and pteropods to ocean warming and acidification. Sci Rep. 9(1):4748. doi: 10.1038/s41598-019-41213-1.

Johnson, M. et al. (2019). Influence of Green Leafy Vegetables in Diets with an Elevated ω-6:ω-3 Fatty Acid Ratio on Rat Blood Pressure, Plasma Lipids, Antioxidant Status and Markers of Inflammation. Nutrients. 11(2). pii: E301. doi: 10.3390/nu11020301.

Statland, J.M. et al. (2019). Rasagiline for amyotrophic lateral sclerosis: A randomized, controlled trial. Muscle Nerve. 59(2):201-207. doi: 10.1002/mus.26335.

kerstrm, B. et al. (2019).  rA1M-035, a Physicochemically Improved Human Recombinant α1-Microglobulin, Has Therapeutic Effects in Rhabdomyolysis-Induced Acute Kidney Injury. Antioxid Redox Signal. 30(4):489-504. doi: 10.1089/ars.2017.7181.

Bednarek, N. et al. (2018). El Nio-Related Thermal Stress Coupled With Upwelling-Related Ocean Acidification Negatively Impacts Cellular to Population-Level Responses in Pteropods Along the California Current System With Implications for Increased Bioenergetic Costs. Frontiers in Marine Science. 5:1-17. doi:10.3389/fmars.2018.00486.

Watanabe, S. et al. (2018). Detection of betacyanin in red-tube spinach (Spinacia oleracea) and its biofortification by strategic hydroponics. PLoS One. 13(9):e0203656. doi: 10.1371/journal.pone.0203656.

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Ferrare, K. et al. (2018). Increase in insulin sensitivity by the association of chicoric acid and chlorogenic acid contained in a natural chicoric acid extract (NCRAE) of chicory ( Cichorium intybus L.) for an antidiabetic effect. Journal of Ethnopharmacology. 215:241–248. doi:10.1016/j.jep.2017.12.035.

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Sheng Cheng, H. (2017). The Ameliorative Effects of a Tocotrienol-Rich Fraction on the AGE-RAGE Axis and Hypertension in High-Fat-Diet-Fed Rats with Metabolic Syndrome. Nutrients. 9(9): 984. doi: 10.3390/nu9090984.

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